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In the hinge-latch hypothesis, the conformation of the Nrf2Keap1 complex is altered upon oxidative stress, implying that induction of target genes is only facilitated by newly synthesized Nrf2

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This color change is caused by the copper-peptide bond itself and serves as a practical visual confirmation that reconstitution was successful

The heterogeneous oxidative profile observed across brain regions likely reflects intrinsic metabolic and cellular differences among neural structures

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