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The uptake and apoptosis test in 3D tumor spheroids To establish 3D tumor spheroids, 50 L of a 1% agarose gel solution (Aladdin) was added into each well of a 96-well plate

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47,48 These synthetic protein preparations exhibit partial or complete amino acid sequence homology with endogenous GLP-1, rendering them resistant to degradation which resulting in a prolonged half-life and heightened biological activity