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Multiple experiments indicate that GCL is the rate-limiting enzyme in the plant glutathione biosynthesis (Foyer et al., 1995, 1997
I., Kim, E
The catch: although GHK is 340.4 daltons and therefore under the 500-dalton passive ceiling, measured permeation through intact human skin is close to zero, because charge and effective hydrodynamic radius matter as much as raw mass
Mechanism involves NO system modulation (homeostatic regulationupregulating when deficient, moderating when excessive), VEGF/VEGFR2 upregulation for angiogenesis, FAK-paxillin pathway activation for cell migration (40% increase per Chang et al.), and EGR-1 transcription factor upregulation for growth factor sensitivity
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