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We administer NAD+ in a wide variety of strengths from 50mg to 750mg
Using a 1:100 ratio of mg tissue to L KME buffer (20 mM MOPS, 120 mM KCl, and 1 mM EGTA, pH 7.4), tissue was homogenized in 2 s bursts separated by a 2 s rest for a total of 10 s (or 3 cycles) at 16,600 rpm using IKA T25 digital Ultra-Turrax homogenizer
Lin and Yao, 2020)
Cell 80 , 796809 e799 (2020)
Hagen TM, Vinarsky V, et al
p21CIP/WAF1 saRNA inhibits proliferative vitreoretinopathy in a rabbit model
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