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After washing with 0.05% Tween-20 in PBS, the secondary antibody ImmunoPure Antibody Anti-Rabbit IgG (#31460, Thermo Scientific) was diluted 1:5000 in 0.05% Tween-20 in PBS, incubated for 1 hour and washed again in 0.05% Tween-20 in PBS

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(4) A Polaska, J Wegner, P Nutbohm, et al (2024) Afamelanotide in protoporphyria and other skin diseases: a review Postepy Dermatology Allergology, 2024 Apr 12, Volume 41 (Issue 2), Pages 149154