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This was patented by Novo Nordisk in 1996 and the first paper on it was published in 2000 (Knudsen, Nielsen, Huusfeldt)

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Thus, incubating human and rodent islets under high glucose conditions results in increased expression of PC1/3 and increased secretion of GLP-1 (101, 108110), while exposure of -cells to the proinflammatory cytokine, interleukin-6, also increased GLP-1 secretion (112)

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